Can biopsy confirmed disease progression give quality data?

Certainly.

NASH research services
NASH partner programs

Contract research services within NASH

We have established and validated a diet-induced obese (DIO) mouse model of non-alcoholic steatohepatitis (NASH) for chronic efficacy studies. The DIO-NASH model exhibits key hallmarks of NASH, including steatosis, inflammation and fibrosis, uniquely identified by pre-study liver biopsy and objectively assessed by individual histological changes from baseline (pre) to study end (post) using the clinical-derived NAFLD activity score and fibrosis stage.

Animals are constantly being diet-induced to allow for monthly conduction of NASH studies, and our pre/-post liver biopsies have proven especially valuable to our customers.

Our main offerings:

  • Diet-induced obese (DIO) mouse model of NASH (DIO-NASH)
  • Obese diet-induced ob/ob mouse model of NASH (ob/ob-NASH)
  • Chronic (8-12 weeks) efficacy studies in mice randomized into study groups based on individual pre-biopsy histological assessment
  • Metabolic assessment (body weight, food intake, glycaemia, insulinemia and glucose tolerance)
  • Histological assessment of hepatic steatosis, inflammation, ballooning degeneration and fibrosis (HE, Sirius Red, IHC) levels
  • Pathologist evaluated NAFLD Activity Score (NAS) including Fibrosis Stage
  • Hepatic collagen content by hydroxyproline assay
  • Hepatic triglyceride and cholesterol content
  • RNA sequencing and bioinformatics
  • Microbiome analysis

Get in touch

Get in touch if you are interested in discussing how to get started with your study.

Michael Feigh

Principal scientist
mfe@gubra.dk
+45 2382 9493

Get in touch

Contact us

Gubra DIO-NASH model

The Gubra diet-induced obesity (DIO) mouse model of NASH (DIO-NASH) is based on wild-type C57BL/6 mice offered a diet high in fat, fructose and cholesterol 26+ weeks prior to pre-biopsy collection. Only animals demonstrating hepatosteatosis and fibrosis are enrolled into study groups.

See data on the histological effects of liraglutide, obeticholic acid (OCA), and elafibranor treatment in DIO-NASH mice

Gubra NASH posters and publications

Gubra benchmark characterization paper
Gubra DIO NASH model characterization paper
Gubra review of preclinical models of NASH
Gubra DIO NASH Benchmark RNA poster 

Gubra ob/ob-NASH model

The Gubra accelerated mouse model of NASH (ob/ob NASH) is based on the leptin-deficient ob/ob mouse offered a diet high in fat, fructose and cholesterol for 12 weeks prior to pre-biopsy collection. Only animals demonstrating hepatosteatosis and fibrosis are enrolled into study groups.

See data on the histological effects of liraglutide, obeticholic acid (OCA), and elafibranor treatment in ob/ob NASH mice

 

“Gubra’s DIO NASH model services are truly best in class.  The ease and speed with which we could initiate studies and receive data was incredibly helpful in advancing our program. In addition, their ability to examine liver histopathology pre-and post-treatment was key to understanding efficacy.”

Brandee Wagner
Director of Biology | Metacrine

Imaging

Gubra now offers state-of-the art 3D imaging technologies to visualize pathogenesis and compound distribution in whole organs with single cell resolution.
See the visualization and read more

Press Release

Find out more about our second partner program with Boeringer Ingelheim Pharma. 

Read more

Careers

We are always looking for talent. Check out our open positions here.

Careers at Gubra

Gubra

Hørsholm Kongevej 11B
2970 Hørsholm
Denmark

info@gubra.dk
+45 3152 ­2650