Dietary intervention improves intestinal permeability and reduces colonic fibro-inflammation in the GAN diet-induced obese and biopsy-confirmed mouse model of MASH 

Background & Aim:

The gut‐liver axis is considered playing an important role in metabolic dysfunction-associated steatohepatitis (MASH). Accordingly, MASH is associated with gut dysbiosis and impaired intestinal barrier function (‘leaky gut’) which may expose the liver to inflammatory microbial products and facilitate pro-fibrotic responses. The Gubra-Amylin NASH (GAN) diet-induced obese (DIO) mouse is an industry-standard translational model of biopsy-confirmed MASH and fibrosis. The present study aimed to assess the impact of dietary intervention (chow reversal) on markers of intestinal permeability and hepatic/colonic inflammation and fibrosis in the biopsy-confirmed GAN DIO-MASH mouse.