Quantitative 3D high-resolution imaging of skeletal muscle morphology in aged lean and diet-induced obese mice
Background & aim: Effective treatment of muscle diseases requires comprehensive, multidimensional assessment of muscle structure, mass, and function. This need is particularly evident in sarcopenia, where age-related loss of muscle mass and quality drives functional decline, increased morbidity, and frailty…
Superior Efficacy and Clinical Translatability of CagriSema Compared to Cagrilintide and Semaglutide in the DIO rats
Background & aim: Long-acting amylin analogues offer mechanistic complementarity to incretin-based therapies for obesity. Accordingly, CagriSema, a fixed-dose combination of the amylin analogue Cagrilintide and the GLP-1 analogue Semaglutide, has demonstrated superior weight loss compared with either agent alone in recent clinical trials…
Differential effects of semaglutide, tirzepatide, and survodutide on energy expenditure in obese mice under thermoneutrality
Background & aim: Obesity pharmacotherapy has significantly improved weight management, with semaglutide (GLP-1 receptor agonist), tirzepatide (dual GLP-1 and GIP receptor agonist) and survodutide (dual glucagon/GLP-1 receptor agonist) demonstrating substantial efficacy in reducing body weight. These drugs primarily act by…
A Multimodal Rat Brain Atlas and Whole-Brain Imaging Pipeline for Mapping Satiety Pathways and Anti-Obesity Drug Effects
Background & aim: Central regulation of appetite and satiety engages neuronal populations distributed across the brain. The identification of novel drug targets, development of anti-obesity therapeutics, and validation of their mode of action benefit from whole-brain imaging. Light-sheet fluorescence…
AI-driven whole-brain cellular profiling using 3D microscopy
Background & aim: Neurodegenerative diseases are characterized by complex, large-scale structural and cellular alterations across the brain. Understanding these changes requires comprehensive, whole-brain analysis at high spatial resolution. Three-dimensional light sheet fluorescence microscopy (3D LSFM) enables rapid imaging of intact…