Time course of hypertensive diabetic kidney disease in uninephrectomized db/db mice
Background & aim: Preclinical models that combine diabetes with hypertension and impaired kidney function is a good tool to recapitulate progressive diabetic kidney disease (DKD). Here, we profiled disease progression in a translational mouse model of DKD, induced by renin…
A short-term adenine diet–induced mouse model of progressive renal dysfunction and chronic kidney disease
Background & aim: Translational models are essential to identify improved treatment options for chronic kidney disease (CKD) patients. However, most preclinical CKD models do not demonstrate reduced glomerular filtration rate (GFR). Here, we characterized the temporal development of functional, histopathological,…
Temporal characterization of kidney injury, inflammation and fibrosis after unilateral ureteral obstruction
Background & aim: Unilateral ureteral obstruction (UUO) is a widely used preclinical model of surgery-induced chronic kidney disease characterized by robust inflammation and tubulointerstitial fibrosis. However, the temporal relationship between tubular injury, inflammatory responses, fibrotic remodeling, and associated transcriptional changes…
Whole-Brain 3D c-Fos Mapping Reveals Distinct Neuropsychiatric Drug Signatures
Background & aim: Psychiatric compounds modulate complex brain networks, making whole-brain assessment of neuronal activity essential for understanding their mechanisms of action. Activity-dependent genes such as c-Fos, and other stimulus-regulated genes (SRGs) provide complementary molecular readouts of neuronal activation, and…
Assessing toxicology in ovaries using a high-throughput 3D imaging and AI-based follicle segmentation and categorization platform
Background & aim: Two-dimensional (2D) histopathology is the current gold-standard for assessing toxicology in ovaries. However, it can be a time-consuming process limited by section-to section variability, subjective interpretation and sampling bias. To overcome these limitations, we have developed a…
Comparative quantitative 3D whole-brain mapping of Tfr1-enabled and unmodified anti-β-amyloid antibody biodistribution
Background & aim: Efficient delivery of anti-β-amyloid (Aβ) antibodies across the blood–brain barrier (BBB) remains a central challenge in Alzheimer’s disease (AD) therapy. Therapeutic Aβ-targeted antibodies engineered for receptor-mediated transcytosis display improved brain exposure and therapeutic efficacy, yet their brain-wide…
Semaglutide attenuates neuroinflammation in male mice
Background & aim: Glucagon-like peptide-1 receptor agonists (GLP-1RAs) have shown promise in preclinical models of neurodegeneration, with emerging evidence suggesting these effects may be driven by modulation of neuroinflammation. However, the cellular mechanisms underlying GLP-1RA effects on neuroinflammation remain poorly…