High-Throughput 3D Imaging and Quantification of Rodent Hindlimb Muscles Using Light Sheet Fluorescence Microscopy
Background & aim: Accurate assessment of muscle mass remains a major challenge in preclinical models of sarcopenia, obesity, and drug-induced atrophy. Traditional readouts such as lean mass from body composition scans lack spatial resolution and anatomical specificity, especially in conditions…
Nephroprotective effects of enalapril in the anti-GBM mouse model of glomerulonephritis
Background & aim: Translational rodent models of glomerulosclerosis are essential in drug discovery for chronic kidney disease (CKD). The anti-glomerular basement membrane (anti-GBM) model in mice represents a clinically relevant model as it exhibits key features of albuminuria and glomerulosclerosis.…
Transferrin receptor-binding blood-brain barrier shuttle enhances brain delivery and efficacy of a therapeutic anti-Aβ antibody
Background & aim: Transferrin receptor-1 (TfR1) transcytosis-mediated delivery of therapeutic monoclonal antibodies across the blood-brain barrier (BBB) is a promising concept in drug development for CNS disorders. In this study, we investigated brain delivery and effects on plaque burden of…
Temporal glomerular gene expression dynamics during disease progression in a mouse model of hypertension-accelerated diabetic kidney disease
Background & aim: The current understanding of diabetic kidney disease (DKD) has significant gaps regarding the underlying pathogenesis. In this study, we aimed to characterize the temporal progression of DKD using a state-of-the-art mouse model of hypertension-accelerated disease, integrating kidney…
Deep learning-enhanced 3D imaging unveils semaglutide impact on cardiac fibrosis
Background & aim: Myocardial fibrosis (MF), a hallmark of structural cardiac remodelling, drives disease progression across most forms of heart failure and plays a central role in heart failure with preserved ejection fraction (HFpEF). Despite its clinical relevance, effective treatments…
Renal transcriptomic profiling reveals beneficial effects of semaglutide and lisinopril in advanced diabetic kidney disease
Background & aim: Obesity, hyperglycemia and hypertension are critical risk factors for development of diabetic kidney disease (DKD). While emerging evidence suggests that glucagon-like peptide-1 receptor (GLP1R) agonists improve cardiovascular and renal outcomes in type 2 diabetes patients, their mode…
Targeting nuclear envelope improves NAFLD Activity Score and shows antifibrotic and hepatoprotective effects superior to Lanifibranor in the GAN diet-induced obese and biopsy-confirmed mouse model of MASH
Background & aim: NLT-101 is a first-in-class compound that functions by proximity engagement with a target in the nuclear envelope that mediates its activity. NLT-101 is in preclinical development for metabolic dysfunction-associated steatohepatitis (MASH) and fibrosis driven indications. The present…