Molecular pharmacology

Get access to robust cellular assays for in vitro pharmacology and functional drug testing.

We offer:

Receptor pharmacology for drug discovery (human/rodent receptors)

• Custom assay design (binding, signaling, functional cellular assays)
• Assay establishment and validation in commercially available and custom transfected cell lines
• Hit-to-lead and lead optimization (agonist or antagonist potency, efficacy)
• Counter-screening on relevant receptors (selectivity)
• Batch activity and stability testing of your dosing compounds on cells expressing relevant rodent receptors to support in vivo studies

Functional cell assays
(Islets of Langerhans, hepatocytes, enterocytes, leukocytes, tissue-derived cell lines)

• Mode-of-action and functionality testing in primary cell systems and tissue-relevant cell lines
• Quantitative pharmacology on cells expressing endogenous receptor levels (EC50/Emax/IC50)
• Linking in vitro and in vivo pharmacology (example: “in vitro PK” estimation)

Technologies

• Signalling: cAMP & cGMP, IPOne, SureFire phosphorylation assays
• Protein expression: ELISA, AlphaLISA/AlphaScreen, MSD multiplex, Western blot
• Gene expression
• Metabolic enzyme activity & proliferation: radioisotope techniques