Can you get 'clinical-like' data from a preclinical model?
In vivo pharmacology
We have expert hands-on experience with a wide variety of commercially available rodent models. Furthermore, we have established and benchmarked a number of in house models, which are always readily available. Our readiness, expertise and flexibility continuously broaden our model catalogue related to our custumers’ demands.
Our technical services include numerous tools to provide you with pivotal information about your compounds efficacy on in-life parameters such as food intake, body composition, energy expenditure and blood biochemistry. Following treatment our expert tissue sampling techniques enable us to perform high quality histopathology, histology/stereology and genetic profiling.
- Online food and water intake and recording of locomotor activity
- Telemetric measurements of heart rate, blood pressure, ECG and blood glucose
- Energy expenditure testing using indirect calorimetry
- Total body fat measurements in live animals or tissue samples using our EchoMRI scanner
- Advanced surgical models: selective vagal deafferentation, central lesions and bariatric surgery
- ICV and brain site-specific compound administration
- Oral/intravenous/intraperitoneal glucose tolerance tests (OGTT, IVGTT, IPGTT)
- Hyperinsulinaemic euglycaemic clamp
- Models of gatrointestinal inflammation (DSS, TNBS)
- Urinary and fecal sampling in metabolic cages
- Cardiac ultrasound
“Gubra’s DIO NASH model services are truly best in class. The ease and speed with which we could initiate studies and receive data was incredibly helpful in advancing our program. In addition, their ability to examine liver histopathology pre-and post-treatment was key to understanding efficacy.”
Director of Biology | Metacrine
Imaging and iDISCO
Imaging and iDISCO
- 3D imaging of whole organs allows unbiased volume determination and IHC on all glomeruli
- Labelled tracers (drug compounds, endothelium-binding lectin)
- Immunofluorescence (iDISCO)
- After imaging, tissue can be processed for conventional histology and immunohistochemistry
Histology, stereology and image analysis
Obtain unbiased quantified tissue data
- Podocyte number (WT-1)
- Glomerular number and volume
- Glomerulus-specific collagen IV
- Tubulointerstitial fibrosis: collagen III
Acute efficacy studies
In an acute efficacy study we offer e.g.
- OGTT/IPGTT/IVGTT and ITT in rats and mice
- Automatized food intake testing
- Conditioned taste aversion testing
- In vitro biology
Chronic efficacy studies
In a chronic efficacy study we offer e.g.
- Body weight, food intake, food preference
- Chronic efficacy testing (7 days to 12+ weeks)
- EchoMRI determination of body fat and tissue fat (e.g. liver)
- Ex vivo assays (TG, Chol, FFAs, Hormones: Leptin, ghrelin, glucagon, insulin, GLP-1, GIP, PYY3-36 etc.)
Examples of Gubra rodent models:
The Gubra accelerated NASH model based on the leptin-deficient ob/ob mice offered a high fat/fructose/cholesterol diet for 12 weeks.
The Gubra pancreatectomized (PX) rat is hyperglycemic from the time of PX and is ideal for anti-diabetic pharmacological testing and for modeling diabetic complications.
The Gubra diet-induced obese (DIO) NASH mouse model enable assessment of various key histological hallmarks of NASH.
Gubra DIO rat
The Gubra diet-induced obesity (DIO) rat is offered a two-choice diet enabling measurements of food preference changes during pharmacological or surgical interventions.
Gubra’s state-of-the-art animal facilities are staffed by trained animal technicians and veterinarians providing professional handling and close monitoring of all research animals.
Gubra is dedicated to ensuring optimal animal welfare and all studies are licensed by the Danish Animal Experimentation Inspectorate and conducted in accordance with the Danish Animal Experimentation Act (BEK nr 12 of 7/01/2016), which is stricter and therefore fully compliant with the European directive (2010/63/EU) and internationally accepted principles for the care and use of laboratory animals.
Gubra applies the 3R principles (replacement, reduction and refinement) to all animal studies and we believe that laboratory animal welfare is an ethical responsibility that encompasses all aspects of animal well-being, including proper housing, clinical and behavioral management, nutrition, disease management and treatment.