Imaging

New technologies such as light sheet microscopy and whole organ immunohistochemistry are revolutionizing our understanding of disease progression and drug distribution.  As one of the first CRO’s in the world, Gubra has implemented these novel technologies into our existing pre-clinical platform, and now offers 3D imaging of intact organs with single cell resolution.

3D imaging is particularly useful when studying complex structures such as the vasculature or neuronal innervation. By injecting fluorescently labelled molecules, such as peptides or antibodies, you can also use the technology to visualize distribution throughout the body and in particular how they access the CNS.

We offer:

• Whole organ imaging (light sheet microscopy)
• In vivo distribution of peptides and antibodies
• Whole organ immunohistochemistry (iDISCO)
• 3D Receptor mapping
• Quantitative image analysis

Light sheet microscopy showing all glomeruli (green) in a kidney from a normal adult mouse. 

Light sheet microscopy showing the bile ducts (green) and vasculature (red) in the liver from a normal adult mouse. 

Light sheet microscopy of a mouse pancreas (low zoom) stained for insulin (blue), smooth muscle actin (red) and tyrosine hydroxylase (green). 

Light sheet microscopy of a mouse pancreas (high zoom) for insulin (blue), smooth muscle actin (red) and tyrosine hydroxylase (green). 

3D imaging of a mouse pancreas stained for Insulin and Smooth muscle actin.

Using high-resolution 3D imaging, Gubra is now offering total quantification of dopaminergic (tyrosine hydroxylase-positive) cell bodies, projections and terminals in the adult mouse brain. This approach enables high-resolution assessment of whole-brain dopaminergic neurotransmission in mouse models of e.g. Parkinson’s disease, schizophrenia, ADHD, and obesity.