Imaging

New technologies such as light sheet microscopy and whole organ immunohistochemistry are revolutionizing our understanding of disease progression and drug distribution.  As one of the first CRO’s in the world, Gubra has implemented these novel technologies into our existing pre-clinical platform, and now offers 3D imaging of intact organs with single cell resolution.

3D imaging is particularly useful when studying complex structures such as the vasculature or neuronal innervation. By injecting fluorescently labelled molecules, such as peptides or antibodies, you can also use the technology to visualize distribution throughout the body and in particular how they access the CNS.

Our main offerings:

• Whole organ imaging (light sheet microscopy)
• In vivo distribution of peptides and antibodies
• Whole organ immunohistochemistry (iDISCO)
• 3D Receptor mapping
• Quantitative image analysis

Quantitative analysis of c-Fos expression

Learn more about quantitative whole brain c-Fos for assessment of compound induced neuronal activation.

Quantitative analysis of glomeruli number and size

See how quantitative whole kidney LSM provides information about glomeruli number of size in diabetic mice.

Quantitative analysis of beta cell mass

See how quantitative whole pancreas LSM provides information about beta cell mass, islet number and size.

Light sheet microscopy showing the bile ducts (green) and vasculature (red) in the liver from a normal adult mouse. 

Using high-resolution 3D imaging, Gubra is now offering total quantification of dopaminergic (tyrosine hydroxylase-positive) cell bodies, projections and terminals in the adult mouse brain. This approach enables high-resolution assessment of whole-brain dopaminergic neurotransmission in mouse models of e.g. Parkinson’s disease, schizophrenia, ADHD, and obesity.

This novel 3D high resolution imaging method visualizes and quantifies myocardial infarction in mice enabling rapid assessment of assessment of ventricular volume and capillary density in a LAD mouse model of cardiac infarction.