Whole organ 3D imaging
Obtain unique endpoints with Gubra 3D imaging platform
Light sheet microscopy and whole organ immunohistochemistry are revolutionizing our understanding of disease progression and drug distribution. Gubra has implemented these novel technologies into our existing pre-clinical platform and now offers 3D imaging of intact organs with single cell resolution.
3D imaging is particularly useful when studying complex structures such as the vasculature or neuronal innervation. By injecting fluorescently labelled molecules, such as peptides or antibodies, you can also use the technology to visualize distribution throughout the body and in particular how they access the CNS.
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Main offerings
Drug induced neuronal activation
- Whole brain cFos
- Unbiased quantification
Neurodegeneration
- Neuron and fiber loss
- Plaque and Tangle volume
- Brain atrophy
Drug distribution
- Peptides
- Small molecules
- Antibodies
Kidney
- Glomerular volume
- Glomerular volume/distribution
Heart and vasculature
- Ventricular volume
- Capillary density
Pancreas
- Beta cell mass
- Beta cell proliferation
- Islet number and islet volume
Other applications
- NASH
- Retinopathy
- Adipose tissue
- Glomerular function
- Receptor mapping
Gubra 3D Experience
To enable fast and efficient data mining of quantitative 3D imaging data, Gubra has developed a web-based 3D imaging data viewer (Gubra 3D Experience, G3DE). This resource provides researchers with a platform for exploring Gubra’s published 3D imaging data in further detail.
Click here to explore individual brain regions of interest, display quantitative c-Fos data and filter for regions with statistically significant response to drug treatment.
3D imaging publications
Quantitative Gubra 3D imaging studies across various organs.
CNS
- Whole-brain activation signatures of weight-lowering drugs
- Whole-brain mapping of amylin-induced neuronal activity in receptor activity–modifying protein (RAMP) 1/3 knockout mice
- Comparative study of voxel-based statistical analysis methods for fluorescently labelled and light sheet imaged whole-brain samples
- An optimized mouse brain atlas for automated mapping and quantification of neuronal activity using iDISCO+ and light sheet fluorescence microscopy
- Whole-brain three-dimensional imaging for quantification of drug targets and treatment effects in mouse models of neurodegenerative diseases
- Quantitative whole-brain 3D imaging of tyrosine hydroxylase-labeled neuron architecture in the mouse MPTP model of Parkinson’s disease
Pancreas
Cardiovascular system
Whole brain cFos
Using a novel reference atlas and improved c-Fos detection algorithms, Gubra offers an unbiased, automated and fast quantitative analysis of drug-induced c-Fos expressions across the whole mouse brain (video left).
For more information on our methods:
A mouse brain atlas for virtual neuroscience
NeuroPedia is our vision of neuroscience in the future. It is an open source database for digital whole-brain maps recorded using light-sheet microscopy. The underlying principle is that all maps registered to the same reference brain are compatible. This will allow researchers to share and compare data from different experiments or overlay gene expression data with neuronal activity or connectivity – virtually!
Kidney
Deep learning computational analysis is applied for kidney-wide quantitation of glomerular volume and numbers (following in vivo administration of flouro-labelled lectin) and other renal structures of interest. Send us the kidney – our 3D imaging platform is applicable to any relevant in vivo kidney disease model (video right).
For more information on our methods:
Heart
High resolution imaging and deep learning computational analysis enables unbiased quantification of cardiovascular morphological changes, including ventricular volume, capillary density and atherosclerotic plaque volume (video left).
Send us the hearts. Our 3D imaging platform is applicable to any relevant in vivo heart disease model.
For more information on our methods:
See animations: LAD endpoints and Plaque burden
Download flyers: 3D quantitation ofcardiovascular disease and 3D quantitation of atherosclerosis
Pancreas
Light-sheet fluorescence microscopy allows for 3D imaging, mapping and quantification of the whole pancreas organ, including complete and unbiased quantifications of beta cell volumes and total number of islets (video left).
Send us the pancreas – our 3D imaging platform is applicable to any relevant in vivo pancreas disease model.
For more information on our methods:
Neurodegeneration
In animal models of Parkinson Disease (PD) estimation of dopaminergic neuron numbers and projections are imperative for model phenotyping and assessment of drug treatment effects (video right).
Send us the brains. Our 3D c-Fos imaging platform is applicable to any relevant in vivo model in the mouse.
For more information on our methods:
