NGS & Bioinformatics

We transform complex omics networks into clear interpretable data

At Gubra we offer advanced molecular analysis based on sequencing on both DNA and RNA. Whether you are doing transcriptomics in form of bulk RNAseq or single-cell RNAseq or microbiomics analysis our sequencing services provide thousands of endpoints from a single sample.

With experience form 10.000+ samples Gubra have gained deep insights into the gene regulations involved in the development of metabolic diseases and their treatment. We offer fast turnover and bioinformatic analysis including interpretation of the data using our expert knowledge.

All elements from design and sample collection to bioinformatic analysis are performed on-site at Gubra with a designated team of molecular biologists and bioinformaticians.

 

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Main technological offerings

mRNA sequencing from fresh frozen tissue
  • Sample collection
  • Sample preparation for sequencing
  • Sequencing using Illumina technology
  • Bioinformatic analysis

See videos and flyers below

Single Cell mRNA sequencing
  • Sample collection
  • Sample preparation for sequencing
  • Barcoding of single cells using 10X™ technology
  • Sequencing using Illumina™ technology
  • Bioinformatic analysis

See videos and flyers below

mRNA sequencing from FFPE tissue
  • RNA isolation from FFPE slides
  • Sample preparation for sequencing
  • Sequencing using Illumina™ technology
  • Bioinformatic analysis

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mRNA sequencing from histologic slides using LCM
  • Sample collection using LCM
  • Sample preparation for sequencing
  • Sequencing using Illumina™ technology
  • Bioinformatic analysis

See videos and flyers below

Microbiome sequencing
  • Sample collection
  • DNA isolation
  • Library preparation
  • Sequencing using Nanopore™ technology
  • Bioinformatic analysis

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Interact with data GubraView
  • Gene by gene expression plots
  • Sorting of data by group, expression and significance
  • Correlation to other end points
  • Disease specific pathways
  • Heatmaps of selected genes

See videos and flyers below

Custom bioinformatic analysis
  • Data mining
  • Pathway analysis

Sequencing Technologies

Investigate the underlying molecular effects of your compound using RNAseq

mRNA sequencing using RNAseq is a powerful addition to your preclinical study. RNAseq will highlight molecular mechanisms of a treatment and is very sensitive and highly reproducible across experiments. Everything from sample collection to analysis or done in-house to ensure high quality data.

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Download data presentation example

Video presentation of RNAseq in GubraView 

Gene expression analysis in metabolic diseases using RNAseq with GubraView

We collect all RNAseq experiments and make them available for our customers realtime in our dataviewer GubraView. GubraView presents data at high resolution and gives a clear overview of all the data. Our bioinformaticians have developed a panel of interactive functionalities allowing customers to raise specific queries enabling deep dives into the data.

More sequencing technologies

Explore gene expression in areas of the brain using LCM combined with mRNA sequencing

Interested in a particular area of a tissue? Using Laser Capture Microscropy (LCM) it is possible to select specific areas or cells from histological samples and perform RNAsequencing on that part of the tissue only.

Perform gene expression analysis on scarce sample material by FFPE mRNA sequencing

Our molecular biology specialists have developed a protocol for extraction of RNA from FFPE blocks. The RNA can be used for RNA sequencing. 

Study specific cell expression profiles using single cell or single nuclei RNAseq

By studying individual cell populations instead of the entire tissue valuable information can be obtained about the main drivers for disease progression.

Explore the microbiome using Nanopore sequencing

The microbiome may impact physiology or act as biomarker both in health and in disease. At Gubra, you can study the microbiome alone (16S rRNA) or in combination with gene expression analysis (RNAseq) from the host.