Gubra video gallery

Webinar on whole brain imaging using light sheet microscopy

Principal Scientist and biologist Jacob Hecksher-Sørensen has immense experience in applying quantitative 3D imaging in early drug discovery. In this webinar, he talks about the basic principles of 3D imaging, how to visualise and quantify intensities and cells in the whole brain. Finally, he talks about how this can be applied to large study groups.

Play webinar: Whole brain imaging using light shee microscopy (opens in new tab)

Welcome to our video gallery where we have collected all of our favorite 3D imaging and stereology videos.

CKD: Mouse model of diabetic nephropathy

See how quantitative whole kidney LSM provides information about glomeruli number of size in diabetic mice.

Quantitative analysis of c-Fos expression

Learn more about quantitative whole brain c-Fos for assessment of compound induced neuronal activation.

Diabetes: Beta cell biology in 3D

See how quantitative whole pancreas LSM provides information about beta cell mass, islet number and size.

CVD: Quantification of capillary density

This novel 3D high resolution imaging method visualizes and quantifies myocardial infarction in mice enabling rapid assessment of assessment of ventricular volume and capillary density in a LAD mouse model of cardiac infarction.

CNS: Mouse model of parkinson’s disease

Gubra is now offer total quantification of dopaminergic (tyrosine hydroxylase-positive) cell bodies, projections and terminals in the adult mouse brain. This approach enables high-resolution assessment of whole-brain dopaminergic neurotransmission in mouse models of e.g. Parkinson’s disease, schizophrenia, ADHD, and obesity.

Inflammation in adipose tissue

Light-sheet microscopy of white adipose tissue from an obese mouse. The sample was stained with antibodies against CD45 (yellow) to label inflammatory cells and SM22 (cyan) to visualize the vasculature.

Wild type kidney from an adult mouse

Light-sheet imaging of a wild type kidney from an adult mouse stained with antibodies against Renin (orange) to label the juxtaglomerular cells and SM22 (cyan) to visualize the vasculature.

Retinal vasculature (white)

Light-sheet imaging of the retinal vasculature (white) in the eye from a wild type adult mouse. The capillaries were labelled in vivo using tomato lectin.

 Healthy liver from an adult mouse

Light-sheet imaging of a healthy liver from an adult mouse stained with antibodies against CK7 (green) to label the bile ducts and alpha-Smooth muscle actin (red) to label the vasculature.

CNS: Mouse model of Parkinson’s disease

 

CVD: 3D quantification – Plaque burden
CVD: 3D quantification – LAD endpoints

 

Diabetes: 3D quantification – Islet numbers and volumes
In vivo distribution of peptides, proteins and antibodies
CKD: Glomerular numbers and volumes
Gene expression analyses

Laser capture microdissection (LCM) enables you to fish out specific cells from heterogeneous organs and gives you a level of precision not possible with manual extraction methods.

An introduction to stereology

Stereology is a practical technique for extracting quantitative information about a 3D structure from measurements made on 2D histological section. It produces results that are unbiased, efficient, and more reliable than other ad hoc quantitative analyses