Ex vivo assays

Customized pre-clinical assay data packages

Get confidence in your study endpoints by tapping into our extensive experience with ex vivo biochemical and immuno-assays combined with broad model knowledge ensuring reproducible pre-clinical assay data packages.

Solutions are custom-made to meet your specific needs using a variety of tissues, blood, urine or stool from your internal experiments or from in vivo studies performed at Gubra.

 

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Main offerings

Biochemical assays
  • Cobas c501 clinical assays
  • Colorimetric assays
Immunoassays
  • ELISA
  • MSD multiplex
  • AlphaLISA
  • Time-resolved FRET
  • Flow cytometry
Automation
  • Biomek FX liquid/sample handling
  • FluidX barcoded tube handling
Bioanalysis
  • Pharmacokinetics (PK)
  • LC-MS/MS
  • Immunoassay
Custom biomarkers
  • Assay development (proteins/phosphoproteins)
  • Scientific and technological evaluation of suggested biomarkers
  • Pre-test in model-relevant matrices for valid and reproducible results

Flow cytometry services

Mapping out drug effects on inflammatory cells

Our flow cytometry services can be customized to meet your needs and applied in a broad range of tissues and disease indications. Applications include immune phenotyping, population dynamics and target engament assays.

We have developed a high throughput method for mapping out hepatic immune cell population in our pre-clinical models of NASH.

Download poster: Obeticholic acid exerts anti-inflammatory action and suppress expansion of hepatic immune cell populations in the Gubra biopsy-confirmed DIO-NASH mouse model.

Indication offerings

Diabetes

 

  • Insulin, c-peptide

  • HbA1c, glucose

  • GLP-1 (active and total)

  • GIP

  • Glucagon

  • Glycogen

  • Ketone bodies

  • DPP-IV activity

Kidney

 

  • Albumin
  • Urea
  • Creatinine
  • Cystatin C
  • KIM-1
  • NGAL
  • TIMP-2
  • IGFBP7
  • Podocalyxin

Obesity and dyslipidaemia

 

  • Triacylglycerides

  • Cholesterol (total, HDL, LDL)

  • Free fatty acids

  • Glycerol

  • Leptin

  • Adiponectin

  • Corticosterone

  • FGF-21

Inflammation

 

  • TNF-α, IL-6, IL-1β

  • MCP-1

  • KC/GRO (CXCL1), MIP-2 (CXCL2)

  • IFN-γ

  • IP-10, IL-10, IL-5

  • Oxidative stress (TBARS, 4-HNE, 8-OHdG)

Liver

 

  • AST, ALT, ALP
  • Collagen/hydroxyproline
  • Bilirubin
  • Total bile acids
  • Cytokeratin 18
  • TIMP-1
  • MMP-9
  • Hyaluronic acid
  • PIIINP
  • MMP-9

Heart

 

  • FABP3

  • MyI3

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