We have more than 50 years of collective experience at Gubra in obesity research.
- DIO models and the majority of commercially available models
- Acute or chronic anorectic properties assessed in real-time food/water/activity monitoring system
- Food preference, taste aversion and pica behavior
- Site-specific compound administration, e.g. i.c.v. or local injections into specific nuclei in the brain
- Total body fat measurements in live animals using our EchoMRI scanner
- Tissue fat measurements in tissue biopsies using small sample EchoMRI scanner
- Energy expenditure using indirect calorimetry
- Obesity target validation
Acute efficacy studies
HM-2/telemetry system data
Anorectic response to weight reducing agents
Individual and simultaneous on-line food intake monitoring in rats and mice (up to 4 rodents per cage). The HM-2 system enables advanced real-time monitoring of food/water intake and locomotor activity.
24 hrs food intake profile in mice
48 hrs food intake profile in rats
POC and chronic efficacy studies
At Gubra, we have developed a diet-induced obesity rat model. One of the hallmarks of our DIO rodent model is our ability to measure food preference changes. Note that liraglutide shifts food preference towards chow. (Similar data have been published by Hansen et al.)
- Chronic efficacy (7 days to 12+ weeks)
- Highly experienced with DIO-animal models (mice, rats, hamsters) or monogenetic models like Zucker obese rats, and ob/ob mice
- Body weight, food intake, food preference
- EchoMRI determination of body fat and tissue fat (e.g. liver)
- Blood analysis (TG, Chol, FFAs, Hormones: Leptin, ghrelin, glucagon, insulin, GLP-1, GIP, PYY3-36 etc)
Body weight change
Total food intake
Palatable Gubra Diet
Mode of action studies
Gubra scientists have been involved in unraveling the central mechanisms under- lying the weight lowering effects of GLP-1 analogues. This has involved numerous high-end mechanistic studies, including vagal deafferentation and area postrema lesion studies, as well as in situ hybridization studies examining gene expression changes in hypotha- lamic areas. A lot of these studies were recently published in the Journal of Clinical Investigation. (Secher, Jelsing 2014 J Clin Invest)
- Selective vagal deafferentation (SDA) and area postrema ablation
- Bariatric surgery
- Blood pressure
- Conditioned taste aversion and pica behavior
- c-Fos – a marker of neuronal activation
Selective vagal deafferentation
Gubra performs advanced surgery to assess the MoA of your compound. Principles of selective vagal deafferentation (SDA) with functional validation demonstrating loss of CCK induced anorexia in SDA rats. Since the procedure spares the efferent innervation of the gut, gut motility is not compromised.
Bariatric surgery - powerful effects on body weight and diabetes remission
Gubra is experienced with bariatric surgeries like vertical sleeve and Roux-en-Y gastric bypass in rats and mice.
Body weight in DIO rats
Looking beyond traditional rodents
In addition to our work with DIO rat models, we are currently investigating using hamsters as an obesity/diabetes/dyslipidaemia model. Hamsters offer a different lipid profile than rats, with a lipid metabolism that is quite similar to humans. DIO hamsters show development of hypercholesterolemia when fed a high fat/high carbohydrate diet with cholesterol supplementation.
Hamsters fed Gubra diet with cholesterol show elevated LDL and HDL and are responsive to the cholesterol lowering agent ezetimibe following 14 days of treatment.
of science Read
We know our results ultimately have to generate value for your company. That’s why we focus on the quality and completeness of the data we provide. We choose the methodology that will yield the most usable results for your company. Scientific research requires care, accuracy and precision. This is the delicate balance we walk. Pursuing methods with scientific merit to give you results that you can use, and that will ultimately generate value for your business.
At Gubra, we are committed to high quality deliverables. Through the strength of our quality systems and processes, from administration and study conduction to data management and reporting, we provide structured, professional and experienced services. All critical processes are governed by Standard Operating Procedures that along with quality control documentation are controlled by our Quality Assurance Group. Consistent training and evaluation processes throughout the organization ensure Gubras dynamic and evolving Quality Assurance System.
keep it simple Read
Just because it’s science, doesn’t mean the research has to be complicated. One of our mantras is the need to get clear, simple answers from research. It’s true, with limited time and resources and infinite pressure to produce results, it can be very tempting to get the most bang for your proverbial buck by trying to include as many endpoints in a study as possible. Yet, what often happens with this approach is that you get inconclusive results that require further investigation, taking more time and using more resources. By taking a straightforward approach to study design, we ensure our customers get clear answers they can use.