Nonalcoholic steatohepatitis (NASH)

Gubra has established and validated a diet-induced obese mouse model of fatty liver disease and nonalcoholic steatohepatitis for chronic efficacy studies. The model exhibit features of steatosis, ballooning degeneration, inflammation and fibrosis, uniquely identified by pre-study liver biopsies and objectively assessed using pathological NAFLD activity score and Fibrosis stage. We also offer an accelerated diet-induced obese mouse model of fatty liver disease and NASH (ob/ob strain).


 
Why collaborate with Gubra on your next NASH study?
1

All our animals are biopsied and randomized based on their individual disease stage.

2

Endpoints include a full histopathological NAFLD activity score with fibrosis stage for individual delta values.

3

High availability of animals.

4

Optional RNAseq and bioinformatics.

Our two NASH models

DIO-NASH model

(26 weeks on diet)

The Gubra DIO NAFLD/NASH model reflects characteristic of fatty liver disease and NASH but is also important for assessment of other metabolic parameters, including body weight, hyperinsulinemia, fasting hyperleptinemia, and impaired glucose tolerance.

See model characteristics here.

Read our model characterization paper here.

ob/ob accelerated NASH model

(12 weeks on diet)

Gubra has established an accelerated NASH model based on the leptin-deficient ob/ob mice fed a high fat/fructose/cholesterol diet.

See model characteristics here.

Read our model characterization paper here.


We also offer:

  • Chronic (8 weeks) efficacy studies in mice randomized into study groups based on individual pre-study biopsy assessments
  • Histological assessment of hepatic steatosis (HE stain) and fibrosis (Sirius Red stain) levels
  • Pathological NAFLD Activity Score (NAS) including fibrosis stage
  • Hepatic collagen content by hydroxyproline assay
  • Hepatic triglyceride and cholesterol content

Global gene expression profiling

– see baseline transcriptional fingerprints of the Gubra DIO and ob/ob NASH model here.

The combination of RNAseq with high-end bioinformatic analyses offer an unique insight into changes at the transcriptional level.


Global Gene Expression Profiling

Let's work together


If you have a study, and you want to know if stereology can be a useful method for gaining accurate results and true quantitative data, give us a call. By applying a methodical approach to sampling, we will help you get useful, usable results.

Contact us

Contact us before you harvest
We often receive inquiries from organizations interested in using stereology, yet they have already harvested the organ, which, in many cases, impacts our ability to apply stereology in a cost- efficient manner. So if you’re interested in (or if you wish to investigate the application of stereology in your study) contact us as early in the process as possible, so we are in the best position to advise you.


The business value
of science Read

We know our results ultimately have to generate value for your company. That’s why we focus on the quality and completeness of the data we provide. We choose the methodology that will yield the most usable results for your company. Scientific research requires care, accuracy and precision. This is the delicate balance we walk. Pursuing methods with scientific merit to give you results that you can use, and that will ultimately generate value for your business.

Quality
assurance Read

At Gubra, we are committed to high quality deliverables. Through the strength of our quality systems and processes, from administration and study conduction to data management and reporting, we provide structured, professional and experienced services. All critical processes are governed by Standard Operating Procedures that along with quality control documentation are controlled by our Quality Assurance Group. Consistent training and evaluation processes throughout the organization ensure Gubras dynamic and evolving Quality Assurance System.

Why we
keep it simple Read

Just because it’s science, doesn’t mean the research has to be complicated. One of our mantras is the need to get clear, simple answers from research. It’s true, with limited time and resources and infinite pressure to produce results, it can be very tempting to get the most bang for your proverbial buck by trying to include as many endpoints in a study as possible. Yet, what often happens with this approach is that you get inconclusive results that require further investigation, taking more time and using more resources. By taking a straightforward approach to study design, we ensure our customers get clear answers they can use.